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The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of fefabthese chimeric vectors into 293T cells and harvest of supernatants 48 h later。

Briefly, FeFAB cells are the modified CRFK cells that express β-galactosidase under the control of the FFV LTR, which is trans-activated by fefabthe FFV Tas protein in relation to。

FeFAB cells (a kind gift from Dr Martin Löchelt, Germany) are modified CRFK cells that are used for FFV infection experiments. FeFAB cells express β-galactosidase under the。

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